Part:BBa_K1919200:Design

J23100 promoter-Constitutive AarC Expression Device

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1578
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 168
  Illegal AgeI site found at 264
  Illegal AgeI site found at 276
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 114
  Illegal BsaI.rc site found at 378
  

Design Notes

The AarC conding sequence with His-tag and T7 terminator were cloned from pET-23-AarC express vector. Promoter and RBS were added by PCR.

Source

The coding sequence of AarC comes from BBa_K1919233.